Basic Information
This note record my ideas/some knowledge in protein study
Salting out of protein
盐析(salting out)是指在蛋白质水溶液中加入中性盐，随着盐浓度增大而使蛋白质沉淀出来的现象。
中性盐是强电解质，溶解度又大，在蛋白质溶液中，
- 一方面与蛋白质争夺水分子，破坏蛋白质胶体颗粒表面的水膜
- 另一方面又大量中和蛋白质颗粒上的电荷，从而使水中蛋白质颗粒积聚而沉淀析出。
常用的中性盐有硫酸铵、氯化钠、硫酸钠等，但以硫酸铵为最多。
得到的蛋白质一般不失活，一定条件下又可重新溶解，故这种沉淀蛋白质的方法在分离、浓缩，贮存、纯化蛋白质的工作中应用极广
Sylvia McLain: How Water Folds Proteins
📺The Physics of Life: How Water Folds Proteins - with Sylvia McLain
View from a physical scientist
- instant life
- real critter
- crustacean
- lobster
- dehydrated
- dubious
- upscale
- triop world
- kenai
- ripped
- Octane
- hexane
- propensity
- ethanol
- sentient
- homeopathy
- metaphorical drink
- pipe cleaner
- spree tiny
- Seamonkeys -> water -> octane/hexane
- Moleculear Structure difference between H2O and C6H14 -> key:Oxygen
- Oxygen has a high propensity to pull negative things towards it, but C and H in hexane eventually end up with an even charge distribution across the molecule which does not exist in water
- Although water is not the only liquid with oxygen(i.e ethanol), water supports life to live -> HOH gets life but CH3CH2OH dose not
- But water in itself is not sentient -> there is a lot of other molecules that make life alive
- Favorite molecule: DNA? -> DNA just a guide/middle management
- Favorite molecule: Protein -> Protein is the main undertaker of activities
- 20 basic amino acids that make your proteins -> same main chain -> diff side chain/functional group
- diversity of life made from these amino acids(i.e 100aa Protein: 20^100 possible output)
- …